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Comparing Cloning Efficiency Of Pgemt And Pgemt Easy Vectors To Topo Ta Cloning Vectors

This post categorized under Vector and posted on June 27th, 2019.
Topo Ta Plasmid Vector: Comparing Cloning Efficiency Of Pgemt And Pgemt Easy Vectors To Topo Ta Cloning Vectors

This Comparing Cloning Efficiency Of Pgemt And Pgemt Easy Vectors To Topo Ta Cloning Vectors has 1112 x 744 pixel resolution with jpeg format. was related topic with this Comparing Cloning Efficiency Of Pgemt And Pgemt Easy Vectors To Topo Ta Cloning Vectors. You can download the Comparing Cloning Efficiency Of Pgemt And Pgemt Easy Vectors To Topo Ta Cloning Vectors picture by right click your mouse and save from your browser.

We evaluated the cloning efficiency of different size PCR products into three T-vector cloning systems. the pGEM-T and pGEM-T Easy Vector Systems and the TOPO TA Cloning System.The TOPO TA Cloning Kit gave a higher percentage of white colonies for this insert size. For larger inserts (13kb) the pGEM-T and pGEM-T Easy Vector Systems yielded significantly more recombinant colonies with a greater percentage of white colonies than the TOPO TA Cloning system. We conclude the pGEM-T and pGEM-T Easy Vectors are a better choice for cloning a broader range This Comparing Cloning Efficiency Of Pgemt And Pgemt Easy Vectors To Topo Ta Cloning Vectors has 1112 x 744 pixel resolution with jpeg format.

The pGEM-T Vector was created by linearizing the pGEM-5Zf() Vector with EcoRV at base 51 and adding a T to both 3-ends. The position of the T is indicated by in the pGEM-T Vector Sequence (.txt).Comparing Cloning Efficiency of the pGEM-T and pGEM-T Easy Vectors to the TOPO TA Cloning Vectors All PubHub Articles Article Figures & Tables Abstract. The pGEM-T Vector Systems are convenient for cloning PCR products. To increase convenience we tested conditions for shortening the ligation time. A 15-minute ligation gave 50% transformants by bluewhite selection with further The pGEM-T Easy Vector Systems are convenient systems to clone PCR products. They offer all of the advantages of the pGEM-T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site.

Comparing Cloning Efficiency of the pGEM-T and pGEM-T Easy Vectors to the TOPO TA Cloning Vectors Citations Roles of 14-3-3 and in regulation of the glucocorticoid receptor transcriptional activation and hepatic gluconeogenesis.The pGEM-T and pGEM-T Easy Systems now allow you to perform ligation reactions in as little as one hour. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation reactions.

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